Postdoc molecular immunology / RNAseq, to develop the next generation of cancer immunotherapy
We are looking for a postdoc with a background molecular biology, preferentially with experience in transcriptomics, single-cell RNAseq or related topics, who is interested to work in a highly motivated multidisciplinary team of researchers to develop the next generation of cancer immunotherapy.
Background of the project
Dendritic cells and T cells can form multicellular complexes (immune rosettes), where several T cells surround a single dendritic cell. Thus far it is largely unknown how these cells communicate with each other in such a multicellular cluster. In an upcoming clinical trial we will harvest such clusters from lymph nodes resected from cancer patients that have been vaccinated with different subsets of dendritic cells. By micromanipulation we will isolate single cells from such clusters, determine their transcriptome, and profile the cell surface by single cell RNAseq and single cell immune-detection techniques. Furthermore, we will analyse DC-T cell clusters formed in vitro and in mice models. We aim to get detailed insight into the composition of such immune cell clusters and the relevant signaling cues that shape early immune responses. This information is of crucial importance for our quest to develop the next generation of cancer vaccines
Candidates should have a PhD degree in molecular cell biology or related fields. The preferred candidate has significant experience in RNA sequencing, (single cell) RNAseq, and experience with bioinformatics tools for analyses of transcriptomic data. Experiences with manipulating immune cells a background in molecular immunology would be a bonus
Radboudumc aims to be at the forefront of the development of innovative, sustainable and affordable healthcare. Our mission is 'to have a significant impact on healthcare'. We believe we can achieve that by providing excellent quality, participatory and personalized healthcare, operational excellence and by working together in sustainable networks. The starting point for this is patients and their quality of life. Throughout all this, patient care, research and education go hand in hand.
To realize our mission, we are searching for colleagues who want to take on this challenge with us in a multidiscplinay team of scientists and clinicians; employees who are excellent in their field of expertise and give it their all by pushing boundaries and providing 'that little bit more'. At Radboudumc, you gain the confidence, receive and take responsibility to successfully make these changes. For the best patient care and the best future of healthcare.
The department of Tumor Immunology is a leading European department in basic- and translational- immunology. It host scientists from over 10 different nationalities and disciplines (Immunology, Molecular Biology, Chemical Immumology, Nanomedicine, Computational Immunology, and Medical Oncology)
The department is embedded in the Radboud Institute for Molecular Life Sciences (RIMLS) in the Netherlands, a leading European research school providing an outstanding research setting for the Radboud University. The RIMLS hosts excellent technology platforms such as genomics, proteomics, and molecular imaging.
Research within the department of Tumor Immulology aims at a better understanding of our immune system using molecular, cell biological, immunological, and computational techniques and is centred around antigen presenting dendritic cells which play an important role in regulating the immune response. An important activity of the department is the translation of basic research into clinical applications. In particular, we are developing novel dendritic cell based vaccines by using a chemical toolbox to treat cancer patients.
Conditions of employment
The position is available for a period of 4 years. After a positive evaluation at 12 months, the contract will be extended. 36 hrs per week, scale 10.
Additional information about the vacancy can be obtained from Prof. dr. Carl Figdor, Carl.Figdor@Radboudumc.nl, +31 (0)24- 361 7600.
If you are interested in the vacancy please send your CV and motivation letter to Sandy.Devue-Budde@radboudumc.nl